Coding
Part:BBa_K4759039:Experience
Designed by: Shiyue Zhao Group: iGEM23_Jiangnan-China (2023-10-05)
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Applications of BBa_K4759039
we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags
(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of
Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
The application of protein tags
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UNIQ703c751ace49c4cd-partinfo-00000000-QINU UNIQ703c751ace49c4cd-partinfo-00000001-QINU